Association between serum miRNAs and gingival gene expression in an obese rat model.
J Periodontal Res
; 57(3): 502-509, 2022 Jun.
Article
em En
| MEDLINE
| ID: mdl-35234284
ABSTRACT
INTRODUCTION:
Recent studies have reported a relationship between periodontitis and obesity; however, the mechanisms of obesity's effects on periodontitis are not well understood. On the other hand, microRNAs (miRNAs) are known to play key roles in the post-transcriptional regulation gene expression by suppressing translation and protein synthesis. We examined the association between obesity-related miRNAs and gene expression in gingival tissue using miRNA-messenger RNA (mRNA) pairing analysis in an obese rat model.METHODS:
Sixteen male Wistar rats aged 8 weeks old were divided into two groups the control group was fed a normal powdered food for 8 weeks, and the obesity group was fed a high-fat diet for 8 weeks. Distance from the cement-enamel junction to the alveolar bone crest of the first molars was measured. miRNA microarray analysis was performed on samples of serum and gingival tissue; the resulting data were used to calculate fold changes in miRNA levels in the obesity group relative to the control group, and miRNA-mRNA pairing analysis was performed to identify mRNAs potentially targeted by miRNAs of interest.RESULTS:
Alveolar bone loss in the obesity group exceeded that in the control group (p = .017). miRNA-mRNA pairing analysis identified an association between 4 miRNAs (miR-759, miR-9a-3p, miR-203b-3p, and miR-878) that were differentially expressed in the obesity and control groups and 7 genes (Ly86, Arid5b, Rgs18, Mlana, P2ry13, Kif1b, and Myt1) expressed in gingival tissue.CONCLUSION:
This study revealed that several miRNAs play an important role in the mechanism of periodontal disease progression induced by the obesity.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Periodontite
/
MicroRNAs
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
J Periodontal Res
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Japão