Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.
Chembiochem
; 23(9): e202200090, 2022 05 04.
Article
em En
| MEDLINE
| ID: mdl-35245408
ABSTRACT
Here we demonstrate a switchable DNA electron-transfer catalyst, enabled by selective destabilization of secondary structure by the denaturant, perchlorate. The system is comprised of two strands, one of which can be selectively switched between a G-quadruplex and duplex or single-stranded conformations. In the G-quadruplex state, it binds hemin, enabling peroxidase activity. This switching ability arises from our finding that perchlorate, a chaotropic Hofmeister ion, selectively destabilizes duplex over G-quadruplex DNA. By varying perchlorate concentration, we show that the DNA structure can be switched between states that do and do not catalyze electron-transfer catalysis. State switching can be achieved in three ways thermally, by dilution, or by concentration.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Quadruplex G
Idioma:
En
Revista:
Chembiochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos