Determining the reliability of rapid SARS-CoV-2 antigen detection in fully vaccinated individuals.
J Clin Virol
; 148: 105119, 2022 03.
Article
em En
| MEDLINE
| ID: mdl-35248992
ABSTRACT
BACKGROUND:
Rapid antigen detection tests (RADT) are commonly used as SARS-CoV-2 diagnostic tests both by medical professionals and laypeople. However, the performance of RADT in vaccinated individuals has not been fully investigated.OBJECTIVES:
RT-qPCR and rapid antigen detection testing were performed to evaluate the performance of the Standard Q COVID-19 Ag Test in detecting SARS-CoV-2 breakthrough infections in vaccinated individuals. STUDYDESIGN:
Two swab specimens, one for RT-qPCR and one for RADT, were collected from vaccinated individuals in an outpatient clinic. For comparison of RADT performance in vaccinated and unvaccinated individuals, a dataset already published by this group was used as reference.RESULTS:
During the delta wave, a total of 696 samples were tested with both RT-qPCR and RADT that included 692 (99.4%) samples from vaccinated individuals. Of these, 76 (11.0%) samples were detected SARS-CoV-2 positive by RT-qPCR and 45 (6.5%) samples by the Standard Q COVID-19 Ag test. Stratified by Ct values, sensitivity of the RADT was 100.0%, 94.4% and 81.1% for Ct ≤ 20 (n=18), Ct ≤ 25 (n=36) and Ct ≤ 30 (n=53), respectively. Samples with Ct values ≥ 30 (n=23) were not detected. Overall RADT specificity was 99.7% and symptom status did not affect RADT performance. Notably, RADT detected 4 out of 4 samples of probable Omicron variant infection based on single nucleotide polymorphism analysis.CONCLUSION:
Our results show that RADT testing remains a valuable tool in detecting breakthrough infections with high viral RNA loads.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vacinação
/
Teste Sorológico para COVID-19
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COVID-19
/
Antígenos Virais
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Virol
Assunto da revista:
VIROLOGIA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Alemanha