AroC, a chorismate synthase, is required for the formation of Edwardsiella tarda biofilms.
Microbes Infect
; 24(5): 104955, 2022.
Article
em En
| MEDLINE
| ID: mdl-35272020
ABSTRACT
Biofilms contribute to the resistance of Edwardsiella tarda to antibiotics and host immunity. AroC in the shikimate pathway produces chorismate to synthesize crucial intermediates such as indole. In this study, the differences between biofilms produced by aroC mutants (â³aroC), wild-type (WT) strains, and â³aroC complementary strains (Câ³aroC) were detected both in vitro with 96-well plates, tubes, or coverslips and in vivo using a mouse model of subcutaneous implants. When examining potential mechanisms, we found that the diameters of the movement rings in soft agar plates and the flagellar sizes and numbers determined by silver staining were all lower for â³aroC than for WT and Câ³aroC. Moreover, qRT-PCR showed that the transcription levels of flagellar synthesis genes, fliA and fliC, were reduced in â³aroC. AroC, FliC, or FliA may accompany the motility of â³aroC strains. In addition, compared with the WT and Câ³aroC, the amounts of indole in â³aroC were significantly decreased. Notably, the formation of biofilms by these strains could be promoted by exogenous indole. Therefore, the aroC gene could affect the biofilm formation of E. tarda concerning its impact on flagella and indole.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fósforo-Oxigênio Liases
/
Edwardsiella tarda
Idioma:
En
Revista:
Microbes Infect
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
MICROBIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China