CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout.
Stem Cell Res
; 60: 102732, 2022 04.
Article
em En
| MEDLINE
| ID: mdl-35279545
ABSTRACT
The receptor tyrosine kinase c-KIT (CD117) has a key role in hematopoiesis and is a marker for endothelial and cardiac progenitor cells. In vivo, deficiency of c-KIT is lethal and therefore using CRISPR/Cas9 editing we generated heterozygous and homozygous c-KIT knockout human embryonic stem cell (ES cell) lines. The c-KIT knockout left ES cell pluripotency unaffected as shown by immunofluorescence and trilineage differentiation potential. Heterozygous and homozygous c-KIT knockouts showed complete loss of exon 17, resulting in ablation of c-KIT protein from the cell surface. c-KIT knockout ES cells provide a valuable tool for further investigating c-KIT biology.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Embrionárias Humanas
Limite:
Humans
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2022
Tipo de documento:
Article