Effects of insulin on the total number of [3H]ouabain binding sites in normal human lymphocytes and after stimulation in vitro with concanavalin A.

ABSTRACT

The effects of insulin in vitro on the total number of [3H]ouabain binding sites were studied in normal and concanavalin A-stimulated human T-lymphocytes after incubation for 18 and 42 h with 0, 10(2) and 10(5) mIU/l of insulin. While no effect on [3H]ouabain binding could be demonstrated in non-stimulated cells, a significant decrease could be produced in concanavalin A-stimulated cells. Mean +/- SD for the total number of [3H]ouabain binding sites per cell after 18 h incubation time with concanavalin A in the absence of insulin was 46,099 +/- 6,620 as compared with 44,783 +/- 8,347 in the presence of 10(2) mIU/l of insulin (non-significant) and 42,406 +/- 7,066 in the presence of 10(5) mIU/l (p = 0.031). The corresponding 42-h values were 47,075 +/- 9,412, 43,761 +/- 9,273 (p = 0.033) and 43,824 +/- 9,312 (p = 0.005).