Detection of cardiac apoptosis by [18F]ML-10 in a mouse model of permanent LAD ligation.
Mol Imaging Biol
; 24(4): 666-674, 2022 08.
Article
em En
| MEDLINE
| ID: mdl-35352214
ABSTRACT
PURPOSE:
The loss of viable cardiac cells and cell death by myocardial infarction (MI) is still a significant obstacle in preventing deteriorating heart failure. Imaging of apoptosis, a defined cascade to cell death, could identify areas at risk. PROCEDURES Using 2-(5-[18F]fluoropentyl)-2-methyl-malonic acid ([18F]ML-10) in autoradiography and positron emission tomography (PET) visualized apoptosis in murine hearts after permanent ligation of the left anterior descending artery (LAD) inducing myocardial infarction (MI). 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) PET imaging localized the infarct area after MI. Histology by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining validated apoptosis in the heart.RESULTS:
Accumulation of [18F]ML-10 was evident in the infarct area after permanent ligation of the LAD in autoradiography and PET imaging. Detection of apoptosis by [18F]ML-10 is in line with the defect visualized by [18F]FDG and the histological approach.CONCLUSION:
[18F]ML-10 could be a suitable tracer for apoptosis imaging in a mouse model of permanent LAD ligation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fluordesoxiglucose F18
/
Infarto do Miocárdio
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Imaging Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Alemanha