Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda.
Vet Med Sci
; 8(4): 1655-1663, 2022 07.
Article
em En
| MEDLINE
| ID: mdl-35420251
ABSTRACT
BACKGROUND:
Abortions cause tremendous economic losses in food-producing animals and may lead to food insecurity.OBJECTIVES:
This study aimed to characterize Brucella spp. and other abortigenic pathogens from aborted tissues of cattle.METHODS:
For cattle, aborted tissues (n = 19) were cultured, and Brucella spp. were detected using the genus-specific 16S-23S ribosomal DNA interspacer region (ITS) assay and speciated using Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR assays. Brucella negative samples were screened using the eight abortigenic pathogens PCR panel. Samples from an abortion outbreak that occurred within a goat tribe were included in this investigation. Sera of females (n = 8) and males (n = 2) were analyzed using the Rose Bengal Test (RBT) and indirect enzyme-linked immunosorbent assay (i-ELISA), while vaginal swabs (n = 3) and aborted tissues (n = 1) were cultured and characterized.RESULTS:
The ITS-PCR detected Brucella DNA in cultures from two aborted tissues of cattle (10.5%, [2/19]), which were identified as B. melitensis (n = 1), and B. abortus (n = 1) using AMOS and Bruce-ladder PCR assays. Campylobacter fetus (n = 7) and Leptospira spp. (n = 4) including co-infections (n = 2) of C. fetus and Leptospira spp. were identified from the Brucella negative samples of cattle. Goats (100.0%, 10/10) were brucellosis seropositive on RBT and i-ELISA. Mixed infections caused by B. melitensis and B. abortus were isolated from the vaginal swabs (n = 3) and aborted tissues (n = 1). DISCUSSION ANDCONCLUSIONS:
This is the first identification of abortion-associated pathogens in aborted cattle indicating the enormous financial losses and a threat to public health. It is therefore essential to include these identified pathogens in the surveillance scheme of veterinary and human services.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Brucella
/
Brucelose
/
Doenças dos Bovinos
/
Doenças das Cabras
/
Leptospira
Limite:
Animals
/
Pregnancy
País/Região como assunto:
Africa
Idioma:
En
Revista:
Vet Med Sci
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
África do Sul