Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.
Food Chem X
; 13: 100207, 2022 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-35498995
ABSTRACT
The present study aimed to explore the possible mechanisms underlying Dendrobium officinale leaf polysaccharides of different molecular weight to alleviate glycolipid metabolic abnormalities, organ dysfunction and gut microbiota dysbiosis of T2D mice. An ultrafiltration membrane was employed to separate two fractions from Dendrobium officinale leaf polysaccharide named LDOP-A and LDOP-B. Here, we present data supporting that oral administration of LDOP-A and LDOP-B ameliorated hyperglycemia, inhibited insulin resistance, reduced lipid concentration, improved ß-cell function. LDOP-A with lower molecular weight exhibited improved effect on diabetes than LDOP-B, concurrent with increased levels of colonic short-chain fatty acids (SCFAs) i.e., butyrate, decreased ratio of Firmicutes to Bacteroidetes phyla, and increased abundance of the gut beneficial bacteria i.e., Lactobacillus, Bifidobacterium and Akkermansia. These results suggest that LDOP-A possesses a stronger effect in ameliorating T2D than LDOP-B which may be related to the distinct improved SCFAs levels produced by the change of intestinal flora microstructure.
AUC, The area under the concentrationtime curve; Dendrobium officinale; FBG, fasting blood glucose; FT-IR, Fourier-transform infrared; GLP-1, glucagon-like peptide-1; GLUT4, glucose transporter type 4; H&E, hematoxylin and eosin; HDL-c, high-density lipoprotein cholesterol; HFD, high-fat diet; HOMA-IR, homeostasis model assessment-insulin resistance; HOMA-ß, ß-cell sensitivity; IC, ion Chromatography; IL-6, interleukin-6; Intestinal microflora; LDL-c, low-density lipoprotein cholesterol; LDOP, Dendrobium officinale leaf polysaccharide; Mw, molecular weight; OGTT, oral glucose tolerance test; OTUs, operational taxonomic units; PAS, periodic acid-Schiff; PYY, peptide YY; Polysaccharide; SCFAs, short chain fatty acids; STZ, streptozotocin; Short-chain fatty acids; T2D, Type 2 Diabetic; TG, triglycerides; TNF-α, tumor necrosis factor-alpha; Type 2 Diabetes
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1
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Food Chem X
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China