Complementation of a polyamine-deficient Escherichia coli mutant by expression of mouse ornithine decarboxylase.
Mol Cell Biol
; 7(1): 564-7, 1987 Jan.
Article
em En
| MEDLINE
| ID: mdl-3550425
ABSTRACT
Mouse ornithine decarboxylase (ODCase) cDNA was expressed at a high level in an Escherichia coli mutant deficient in polyamine biosynthesis. The expression of mouse ornithine decarboxylase relieved the dependence of the mutant on an exogenous source of polyamines, presumably by providing putrescine, the product of the enzyme. The effect on the enzymatic activity of deletions that removed carboxy-terminal amino acids of the protein was determined.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ornitina Descarboxilase
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Poliaminas
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Escherichia coli
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Genes
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Mutação
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1987
Tipo de documento:
Article