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Comparative Analyses of Scylla olivacea Gut Microbiota Composition and Function Suggest the Capacity for Polyunsaturated Fatty Acid Biosynthesis.
Lau, Nyok-Sean; Ting, Seng Yeat; Sam, Ka-Kei; M, Janaranjani; Wong, Swe Cheng; Wu, Xugan; Waiho, Khor; Fazhan, Hanafiah; Shu-Chien, Alexander Chong.
Afiliação
  • Lau NS; Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia.
  • Ting SY; Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia.
  • Sam KK; Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia.
  • M J; Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia.
  • Wong SC; Centre For Marine & Coastal Studies, Universiti Sains Malaysia, 11800, Penang, Malaysia.
  • Wu X; Centre for Research On Environmental Ecology and Fish Nutrition of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China.
  • Waiho K; Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia.
  • Fazhan H; Higher Institution Centre of Excellence (HICOE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
  • Shu-Chien AC; Higher Institution Centre of Excellence (HICOE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Microb Ecol ; 86(1): 575-588, 2023 Jul.
Article em En | MEDLINE | ID: mdl-35618944
ABSTRACT
Although numerous studies in aquatic organisms have linked lipid metabolism with intestinal bacterial structure, the possibility of the gut microbiota participating in the biosynthesis of beneficial long-chain polyunsaturated fatty acid (LC-PUFA) remains vague. We profiled the gut microbiota of the mud crab Scylla olivacea fed with either a LC-PUFA rich (FO) or a LC-PUFA-poor but C18-PUFA substrate-rich (LOCO) diet. Additionally, a diet with a similar profile as LOCO but with the inclusion of an antibiotic, oxolinic acid (LOCOAB), was also used to further demarcate the possibility of LC-PUFA biosynthesis in gut microbiota. Compared to diet FO treatment, crabs fed diet LOCO contained a higher proportion of Proteobacteria, notably two known taxonomy groups with PUFA biosynthesis capacity, Vibrio and Shewanella. Annotation of metagenomic datasets also revealed enrichment in the KEGG pathway of unsaturated fatty acid biosynthesis and polyketide synthase-like system sequences with this diet. Intriguingly, diet LOCOAB impeded the presence of Vibrio and Shewanella and with it, the function of unsaturated fatty acid biosynthesis. However, there was an increase in the function of short-chain fatty acid production, accompanied by a shift towards the abundance of phyla Bacteroidota and Spirochaetota. Collectively, these results exemplified bacterial communities and their corresponding PUFA biosynthesis pathways in the microbiota of an aquatic crustacean species.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Braquiúros / Microbioma Gastrointestinal Limite: Animals Idioma: En Revista: Microb Ecol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Malásia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Braquiúros / Microbioma Gastrointestinal Limite: Animals Idioma: En Revista: Microb Ecol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Malásia