Your browser doesn't support javascript.
loading
Impact of e-cigarette aerosol on primary human alveolar epithelial type 2 cells.
Wick, Katherine D; Fang, Xiaohui; Maishan, Mazharul; Matsumoto, Shotaro; Spottiswoode, Natasha; Sarma, Aartik; Simoneau, Camille; Khakoo, Manisha; Langelier, Chaz; Calfee, Carolyn S; Gotts, Jeffrey E; Matthay, Michael A.
Afiliação
  • Wick KD; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Fang X; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Maishan M; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Matsumoto S; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Spottiswoode N; Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, California.
  • Sarma A; Division of Pulmonary and Critical Care, Department of Medicine, University of California, San Francisco, California.
  • Simoneau C; Gladstone Institutes, University of California, San Francisco, California.
  • Khakoo M; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Langelier C; Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, California.
  • Calfee CS; Chan Zuckerberg Biohub, San Francisco, California.
  • Gotts JE; Cardiovascular Research Institute, University of California, San Francisco, California.
  • Matthay MA; Division of Pulmonary and Critical Care, Department of Medicine, University of California, San Francisco, California.
Am J Physiol Lung Cell Mol Physiol ; 323(2): L152-L164, 2022 08 01.
Article em En | MEDLINE | ID: mdl-35670478
ABSTRACT
Electronic cigarettes (e-cigarettes) are designed to simulate combustible cigarette smoking and to aid in smoking cessation. Although the number of e-cigarette users has been increasing, the potential health impacts and biological effects of e-cigarettes are still not fully understood. Previous research has focused on the biological effects of e-cigarettes on lung cancer cell lines and distal airway epithelial cells; however, there have been few published studies on the effect of e-cigarettes on primary lung alveolar epithelial cells. The primary purpose of this study was to investigate the direct effect of e-cigarette aerosol on primary human lung alveolar epithelial type 2 (AT2) cells, both alone and in the presence of viral infection. The Melo-3 atomizer caused direct AT2 cell toxicity, whereas the more popular Juul pod's aerosol did not have a detectable cytotoxic effect on AT2 cells. Juul nicotine aerosol also did not increase short-term susceptibility to viral infection. However, 3 days of exposure upregulated genes central to the generation of reactive oxygen species, lipid peroxidation, and carcinogen metabolism and downregulated key innate immune system genes related to cytokine and chemokine signaling. These findings have implications for the potentially injurious impact of long-term use of popular low-power e-cigarette pods on the human alveolar epithelium. Gene expression data might be an important endpoint for evaluating the potential harmful effects of vaping devices that do not cause overt toxicity.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas Eletrônicos de Liberação de Nicotina / Vaping Limite: Humans Idioma: En Revista: Am J Physiol Lung Cell Mol Physiol Assunto da revista: BIOLOGIA MOLECULAR / FISIOLOGIA Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas Eletrônicos de Liberação de Nicotina / Vaping Limite: Humans Idioma: En Revista: Am J Physiol Lung Cell Mol Physiol Assunto da revista: BIOLOGIA MOLECULAR / FISIOLOGIA Ano de publicação: 2022 Tipo de documento: Article