A versatile cortical pattern-forming circuit based on Rho, F-actin, Ect2, and RGA-3/4.
J Cell Biol
; 221(8)2022 08 01.
Article
em En
| MEDLINE
| ID: mdl-35708547
ABSTRACT
Many cells can generate complementary traveling waves of actin filaments (F-actin) and cytoskeletal regulators. This phenomenon, termed cortical excitability, results from coupled positive and negative feedback loops of cytoskeletal regulators. The nature of these feedback loops, however, remains poorly understood. We assessed the role of the Rho GAP RGA-3/4 in the cortical excitability that accompanies cytokinesis in both frog and starfish. RGA-3/4 localizes to the cytokinetic apparatus, "chases" Rho waves in an F-actin-dependent manner, and when coexpressed with the Rho GEF Ect2, is sufficient to convert the normally quiescent, immature Xenopus oocyte cortex into a dramatically excited state. Experiments and modeling show that changing the ratio of RGA-3/4 to Ect2 produces cortical behaviors ranging from pulses to complex waves of Rho activity. We conclude that RGA-3/4, Ect2, Rho, and F-actin form the core of a versatile circuit that drives a diverse range of cortical behaviors, and we demonstrate that the immature oocyte is a powerful model for characterizing these dynamics.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Citoesqueleto
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Proteínas Proto-Oncogênicas
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Actinas
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Proteínas rho de Ligação ao GTP
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Proteínas Ativadoras de GTPase
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2022
Tipo de documento:
Article