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Haplotype variants of Sr46 in Aegilops tauschii, the diploid D genome progenitor of wheat.
Athiyannan, Naveenkumar; Long, Yunming; Kang, Houyang; Chandramohan, Sutha; Bhatt, Dhara; Zhang, Qijun; Klindworth, Daryl L; Rouse, Matthew N; Friesen, Timothy L; McIntosh, Robert; Zhang, Peng; Forrest, Kerrie; Hayden, Mathew; Patpour, Mehran; Hovmøller, Mogens S; Hickey, Lee T; Ayliffe, Michael; Cai, Xiwen; Lagudah, Evans S; Periyannan, Sambasivam; Xu, Steven S.
Afiliação
  • Athiyannan N; CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
  • Long Y; Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
  • Kang H; Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
  • Chandramohan S; Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
  • Bhatt D; Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, 611130, Sichuan, China.
  • Zhang Q; CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
  • Klindworth DL; CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
  • Rouse MN; Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
  • Friesen TL; USDA-ARS, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Centre, 1616 Albrecht Blvd. North, Fargo, ND, 58102, USA.
  • McIntosh R; USDA-ARS, Cereal Disease Laboratory and Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.
  • Zhang P; USDA-ARS, Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Centre, 1616 Albrecht Blvd. North, Fargo, ND, 58102, USA.
  • Forrest K; Plant Breeding Institute, University of Sydney, Cobbitty, NSW, 2570, Australia.
  • Hayden M; Plant Breeding Institute, University of Sydney, Cobbitty, NSW, 2570, Australia.
  • Patpour M; AgriBio, LaTrobe R&D Park, Bundoora, Australia.
  • Hovmøller MS; AgriBio, LaTrobe R&D Park, Bundoora, Australia.
  • Hickey LT; Global Rust Reference Centre, Aarhus University, Forsogsvej 1, 4200, Slagelse, Denmark.
  • Ayliffe M; Global Rust Reference Centre, Aarhus University, Forsogsvej 1, 4200, Slagelse, Denmark.
  • Cai X; Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, 4072, Australia.
  • Lagudah ES; CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
  • Periyannan S; USDA-ARS, Wheat, Sorghum & Forage Research Unit, and Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA.
  • Xu SS; CSIRO Agriculture and Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
Theor Appl Genet ; 135(8): 2627-2639, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35748907
ABSTRACT
KEY MESSAGE Stem rust resistance genes, SrRL5271 and Sr672.1 as well as SrCPI110651, from Aegilops tauschii, the diploid D genome progenitor of wheat, are sequence variants of Sr46 differing by 1-2 nucleotides leading to non-synonymous amino acid substitutions. The Aegilops tauschii (wheat D-genome progenitor) accessions RL 5271 and CPI110672 were identified as resistant to multiple races (including the Ug99) of the wheat stem rust pathogen Puccinia graminis f. sp. tritici (Pgt). This study was conducted to identify the stem rust resistance (Sr) gene(s) in both accessions. Genetic analysis of the resistance in RL 5271 identified a single dominant allele (SrRL5271) controlling resistance, whereas resistance segregated at two loci (SR672.1 and SR672.2) for a cross of CPI110672. Bulked segregant analysis placed SrRL5271 and Sr672.1 in a region on chromosome arm 2DS that encodes Sr46. Molecular marker screening, mapping and genomic sequence analysis demonstrated SrRL5271 and Sr672.1 are alleles of Sr46. The amino acid sequence of SrRL5271 and Sr672.1 is identical but differs from Sr46 (hereafter referred to as Sr46_h1 by following the gene nomenclature in wheat) by a single amino acid (N763K) and is thus designated Sr46_h2. Screening of a panel of Ae. tauschii accessions identified an additional allelic variant that differed from Sr46_h2 by a different amino acid (A648V) and was designated Sr46_h3. By contrast, the protein encoded by the susceptible allele of Ae. tauschii accession AL8/78 differed from these resistance proteins by 54 amino acid substitutions (94% nucleotide sequence gene identity). Cloning and complementation tests of the three resistance haplotypes confirmed their resistance to Pgt race 98-1,2,3,5,6 and partial resistance to Pgt race TTRTF in bread wheat. The three Sr46 haplotypes, with no virulent races detected yet, represent a valuable source for improving stem resistance in wheat.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Basidiomycota / Aegilops Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Basidiomycota / Aegilops Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Austrália