Aspidopterys obcordata vine inulin fructan affects urolithiasis by modifying calcium oxalate crystallization.
Carbohydr Polym
; 294: 119777, 2022 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-35868792
ABSTRACT
Aspidopterys obcordata vine is a Chinese Dai ethnic herb used to treat urolithiasis. However, the material basis and underlying mechanisms remain undefined. In this study, a 2.3 kD inulin-like A. obcordata fructan (AOFOS) was isolated by size exclusion column chromatography and characterized by ultrahigh-performance liquid chromatography-ion trap-time of flight mass spectrometry (UPLC-IT-TOF-MS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and high-performance gel permeation chromatography (HGPC). In addition, AOFOS showed unique anti-urolithiasis activity in Drosophila kidney stone models. Mechanism study indicated that AOFOS reduced the size of calcium oxalate crystals by inhibiting the formation of large size crystals and the generation rate of calcium oxalate crystals as well as the crystal form conversion from calcium oxalate monohydrate (COM) to calcium oxalate dihydrate (COD).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cálculos Renais
/
Malpighiaceae
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China