Your browser doesn't support javascript.
loading
Hybrid Polyelectrolyte Nanocomplexes for Non-Viral Gene Delivery with Favorable Efficacy and Safety Profile.
Maiorano, Gabriele; Guido, Clara; Russo, Annamaria; Giglio, Andrea; Rizzello, Loris; Testini, Mariangela; Cortese, Barbara; D'Amone, Stefania; Gigli, Giuseppe; Palamà, Ilaria Elena.
Afiliação
  • Maiorano G; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
  • Guido C; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
  • Russo A; Department of Mathematics and Physics, University of Salento, Monteroni Street, 73100 Lecce, Italy.
  • Giglio A; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
  • Rizzello L; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
  • Testini M; Department of Pharmaceutical Sciences (DISFARM), University of Milan, G. Balzaretti 9 Street, 20133 Milan, Italy.
  • Cortese B; National Institute of Molecular Genetics (INGM), Francesco Sforza 35 Street, 20122 Milan, Italy.
  • D'Amone S; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
  • Gigli G; Nanotechnology Institute of National Research Council, CNR-NANOTEC, c/o La Sapienza University, Piazzale Aldo Moro, 00185 Rome, Italy.
  • Palamà IE; Nanotechnology Institute of National Research Council, CNR-NANOTEC, Monteroni Street, 73100 Lecce, Italy.
Pharmaceutics ; 14(7)2022 Jun 21.
Article em En | MEDLINE | ID: mdl-35890206
ABSTRACT
The development of nanovectors for precise gene therapy is increasingly focusing on avoiding uncontrolled inflammation while still being able to effectively act on the target sites. Herein, we explore the use of non-viral hybrid polyelectrolyte nanocomplexes (hPECs) for gene delivery, which display good transfection efficacy coupled with non-inflammatory properties. Monodisperse hPECs were produced through a layer-by-layer self-assembling of biocompatible and biodegradable polymers. The resulting nanocomplexes had an inner core characterized by an EGFP-encoding plasmid DNA (pDNA) complexed with linear polyethyleneimine or protamine (PEI or PRM) stabilized with lecithin and poly(vinyl alcohol) (PVA) and an outer layer consisting of medium-molecular-weight chitosan (CH) combined with tripolyphosphate (TPP). PEI- and PRM-hPECs were able to efficiently protect the genetic cargo from nucleases and to perform a stimuli-responsive release of pDNA overtime, thus guaranteeing optimal transfection efficiency. Importantly, hPECs revealed a highly cytocompatible and a non-inflammatory profile in vitro. These results were further supported by evidence of the weak and unspecific interactions of serum proteins with both hPECs, thus confirming the antifouling properties of their outer shell. Therefore, these hPECs represent promising candidates for the development of effective, safe nanotools for gene delivery.
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Pharmaceutics Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Pharmaceutics Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Itália