Investigation of urine metabolome of BALB/c mouse infected with an avirulent strain of Toxoplasma gondii.
Parasit Vectors
; 15(1): 271, 2022 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-35906695
ABSTRACT
BACKGROUND:
The protozoan parasite Toxoplasma gondii is a major concern for human and animal health. Although the metabolic understanding of toxoplasmosis has increased in recent years, the analysis of metabolic alterations through noninvasive methodologies in biofluids remains limited.METHODS:
Here, we applied liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics and multivariate statistical analysis to analyze BALB/c mouse urine collected from acutely infected, chronically infected and control subjects.RESULTS:
In total, we identified 2065 and 1409 metabolites in the positive electrospray ionization (ESI +) mode and ESI - mode, respectively. Metabolomic patterns generated from principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) score plots clearly separated T. gondii-infected from uninfected urine samples. Metabolites with altered levels in urine from T. gondii-infected mice revealed changes in pathways related to amino acid metabolism, fatty acid metabolism, and nicotinate and nicotinamide metabolism.CONCLUSIONS:
This is the first study to our knowledge on urine metabolic profiling of BALB/c mouse with T. gondii infection. The urine metabolome of infected mouse is distinctive and has value in the understanding of Toxoplasmosis pathogenesis and improvement of treatment.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxoplasma
/
Toxoplasmose
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Parasit Vectors
Ano de publicação:
2022
Tipo de documento:
Article