Theoretical analysis of a translocation-like model with saturable kinetics.
Biochem J
; 242(3): 835-9, 1987 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-3593279
ABSTRACT
A theoretical analysis of the initial rates of product appearance in both compartments of a specifically designed diffusion cell separated by an asymmetrical enzyme membrane is presented. Variable substrate concentrations and different substrate diffusional limitations were considered. Our analysis shows that, under specific conditions, not only a product accumulation occurs in the compartment opposite to that in which the reaction takes place, but that substrate saturable kinetics can be obtained. These product translocation-like kinetics appear similar to those observed with translocation processes reported for biological situations. For such phenomena, a key role of the diffusion layer surrounding a bioactive surface is proposed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sacarase
/
Enzimas Imobilizadas
/
Modelos Biológicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1987
Tipo de documento:
Article