Colorimetric/SERS dual-channel nanoprobe for reactive oxygen species monitoring in elucidating the mechanism of chemotherapeutic drugs action on cancer cells.
Mikrochim Acta
; 189(9): 351, 2022 08 25.
Article
em En
| MEDLINE
| ID: mdl-36008738
ABSTRACT
Reactive oxygen species (ROS) are involved in drug-induced cytotoxicity by regulating cell signaling, inducing oxidative stress, and damaging the DNA and proteins. Examining ROS production in cells under the stimulation of chemotherapeutic drugs is of great importance for understanding the ROS roles and identifying the mechanism of drug-induced cytotoxicity. Here, a silver/gold (Ag/Au) nanoshell-based colorimetric and surface-enhanced Raman spectroscopy (SERS) dual-response nanoprobe was proposed for ROS sensing on the basis of Ag etching. In this study, as a kind of ROS, hydrogen peroxide (H2O2) was detected by the prepared nanoprobe. The linear ranges of 0.5-100 µM with a limit of detection (LOD) of 0.343 µM for the colorimetric determination and 1-50 µM with LOD of 0.294 µM for SERS determination were achieved. The detection of cellular ROS concentration after stimulation by cisplatin, paclitaxel, doxorubicin, and 5-fluorouracil was validated by the nanoprobe. The nanoprobe could also be used to detect the signal pathway of ROS production by cisplatin stimulation. This study provided a simple and novel dual-response nanoplatform for detecting and monitoring ROS in cells, which holds great potential for elucidating the mechanism of occurrence and treatment of ROS-involved diseases.
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MEDLINE
Assunto principal:
Nanopartículas Metálicas
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Neoplasias
Idioma:
En
Revista:
Mikrochim Acta
Ano de publicação:
2022
Tipo de documento:
Article