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AGO104 is a RdDM effector of paramutation at the maize b1 locus.
Aubert, Juliette; Bellegarde, Fanny; Oltehua-Lopez, Omar; Leblanc, Olivier; Arteaga-Vazquez, Mario A; Martienssen, Robert A; Grimanelli, Daniel.
Afiliação
  • Aubert J; DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France.
  • Bellegarde F; DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France.
  • Oltehua-Lopez O; Universidad Veracruzana, INBIOTECA, Xalapa, Veracruz, Mexico.
  • Leblanc O; DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France.
  • Arteaga-Vazquez MA; Universidad Veracruzana, INBIOTECA, Xalapa, Veracruz, Mexico.
  • Martienssen RA; Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, New York, United States of America.
  • Grimanelli D; DIADE, University of Montpellier, CIRAD, IRD, Montpellier, France.
PLoS One ; 17(8): e0273695, 2022.
Article em En | MEDLINE | ID: mdl-36040902
Although paramutation has been well-studied at a few hallmark loci involved in anthocyanin biosynthesis in maize, the cellular and molecular mechanisms underlying the phenomenon remain largely unknown. Previously described actors of paramutation encode components of the RNA-directed DNA-methylation (RdDM) pathway that participate in the biogenesis of 24-nucleotide small interfering RNAs (24-nt siRNAs) and long non-coding RNAs. In this study, we uncover an ARGONAUTE (AGO) protein as an effector of the RdDM pathway that is in charge of guiding 24-nt siRNAs to their DNA target to create de novo DNA methylation. We combined immunoprecipitation, small RNA sequencing and reverse genetics to, first, validate AGO104 as a member of the RdDM effector complex and, then, investigate its role in paramutation. We found that AGO104 binds 24-nt siRNAs involved in RdDM, including those required for paramutation at the b1 locus. We also show that the ago104-5 mutation causes a partial reversion of the paramutation phenotype at the b1 locus, revealed by intermediate pigmentation levels in stem tissues. Therefore, our results place AGO104 as a new member of the RdDM effector complex that plays a role in paramutation at the b1 locus in maize.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metilação de DNA / Proteínas de Arabidopsis Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Metilação de DNA / Proteínas de Arabidopsis Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França