Virus reduction neutralization test and LI-COR microneutralization assay bridging and WHO international standard calibration studies for respiratory syncytial virus.
Bioanalysis
; 14(15): 1067-1079, 2022 Aug.
Article
em En
| MEDLINE
| ID: mdl-36125040
ABSTRACT
Background:
Respiratory syncytial virus (RSV) vaccine is an unmet medical need. The virus reduction neutralization test (VRNT) was developed to replace the LI-COR microneutralization assay to measure RSV neutralization titers.Methods:
A bridging study using selected V171 phase I samples and calibration studies using the WHO international standard antiserum to RSV were performed to compare VRNT and LI-COR.Results:
From the bridging study, we showed good concordance between VRNT and LI-COR titers, and similar post-/prevaccination titer ratios. From the calibration studies, we can convert VRNT and LI-COR titers into similar IU/ml.Conclusion:
The VRNT and LI-COR microneutralization assay correlate well and the titers can be standardized as similar IU/ml, enabling direct comparison of titers from different assays.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus Sinciciais Respiratórios
/
Vacinas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos