Stress granules and mTOR are regulated by membrane atg8ylation during lysosomal damage.
J Cell Biol
; 221(11)2022 11 07.
Article
em En
| MEDLINE
| ID: mdl-36179369
ABSTRACT
We report that lysosomal damage is a hitherto unknown inducer of stress granule (SG) formation and that the process termed membrane atg8ylation coordinates SG formation with mTOR inactivation during lysosomal stress. SGs were induced by lysosome-damaging agents including SARS-CoV-2ORF3a, Mycobacterium tuberculosis, and proteopathic tau. During damage, mammalian ATG8s directly interacted with the core SG proteins NUFIP2 and G3BP1. Atg8ylation was needed for their recruitment to damaged lysosomes independently of SG condensates whereupon NUFIP2 contributed to mTOR inactivation via the Ragulator-RagA/B complex. Thus, cells employ membrane atg8ylation to control and coordinate SG and mTOR responses to lysosomal damage.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Helicases
/
RNA Helicases
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Família da Proteína 8 Relacionada à Autofagia
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2022
Tipo de documento:
Article