Your browser doesn't support javascript.
loading
The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice.
Iwasaki, Wakana; Yoshida, Ryo; Liu, Hongxia; Hori, Shota; Otsubo, Yuki; Tanaka, Yasutake; Sato, Masao; Ishizuka, Satoshi.
Afiliação
  • Iwasaki W; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Yoshida R; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Liu H; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Hori S; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Otsubo Y; Faculty of Agriculture, Kyushu University, Fukuoka, 819-0385, Japan.
  • Tanaka Y; Faculty of Agriculture, Kyushu University, Fukuoka, 819-0385, Japan.
  • Sato M; Faculty of Agriculture, Kyushu University, Fukuoka, 819-0385, Japan.
  • Ishizuka S; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan. zuka@chem.agr.hokudai.ac.jp.
Sci Rep ; 12(1): 16707, 2022 10 06.
Article em En | MEDLINE | ID: mdl-36202928
In our previous study, enterohepatic 12α-hydroxylated (12α) bile acid (BA) levels were found to be correlated with hepatic triacylglycerol concentration in rats fed high-fat (HF) diet. Since BA composition is diverse depending on animal species, we evaluated whether such a relationship is applicable in mice in response to an HF diet. C57BL/6JJmsSLC (B6) male mice were fed HF diet for 13 weeks and analyzed for triacylglycerol, cholesterol, oxysterols, and other metabolites in the liver. The BA composition was determined in the liver, small intestinal contents, portal plasma, aortic plasma, and feces. Neutral sterols were also measured in the feces. The ratio of 12α BA/non-12 BA increased in the liver, portal plasma, small intestinal contents, and feces of HF-fed B6 mice. Moreover, a positive correlation was observed between the ratio of fecal 12α BAs/non-12 BAs and hepatic triacylglycerol concentration. The concentration of 7α-hydroxycholesterol was increased in the liver of HF-fed B6 mice, whereas no increase was observed in the hepatic expression of cytochrome P450 family 7 subfamily A member 1. The present study showed that the ratio of 12α BA/non-12 BA in feces is closely associated with hepatic triacylglycerol accumulation in B6 mice fed HF diet.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos e Sais Biliares / Oxisteróis Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos e Sais Biliares / Oxisteróis Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão