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Improved detection of hepatitis C virus-positive blood donors and determination of infection status.
Nishiya, Anna S; de Almeida-Neto, Cesar; Witkin, Steven S; Ferreira, Suzete C; Salles, Nanci A; Nogueira, Fátima A H; Oliveira, Claudia Di Lorenzo; Rocha, Vanderson; Mendrone Júnior, Alfredo.
Afiliação
  • Nishiya AS; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil.
  • de Almeida-Neto C; Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM-31), Department of Hematology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
  • Witkin SS; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil.
  • Ferreira SC; Disciplina de Ciências Médicas, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil.
  • Salles NA; Instituto de Medicina Tropical da Universidade de São Paulo, São Paulo, Brazil.
  • Nogueira FAH; Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, New York, USA.
  • Oliveira CDL; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil.
  • Rocha V; Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM-31), Department of Hematology, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
  • Mendrone Júnior A; Fundação Pró-Sangue Hemocentro de São Paulo, São Paulo, Brazil.
Transfus Med ; 33(2): 159-164, 2023 Apr.
Article em En | MEDLINE | ID: mdl-36251615
BACKGROUND: In low-risk populations, variability in the sensitivity of current serological tests for Hepatitis C virus (HCV) blood donor screening may lead to the presence of false-positive results. This contributes to the unnecessary loss of blood donor samples as well as to difficulty in accurate donor counselling. The present study determined the optimal cut-off value of a chemiluminescent immunoassay for identification of HCV-reactive blood donors. STUDY DESIGN AND METHODS: In a retrospective cross-sectional analysis of 193 973 blood donations, 578 samples that were positive for HCV antibody in a chemiluminescent immunoassay and/or RNA screening tests were identified. Blood from 379 of these positive samples was available for retesting by a second confirmatory HCV immunoassay followed by a receiver operating characteristic (ROC) curve analysis. Donors were also recalled for a new analysis. RESULTS: Only 71 (18.7%) blood samples remained HCV-positive upon retesting, while 233 (61.5%) now tested negative and 75 (19.8%) yielding indeterminate results. A signal to cutoff ratio ≥4.32 was determined as the best differential threshold between a positive and negative result, increasing the positive predictive value from 27.3% to 66.7%. CONCLUSION: Using a higher threshold for an HCV-positive blood sample enhances the chemiluminescent immunoassay screening test´s accuracy and helps to improve donor counselling and notification processes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doadores de Sangue / Hepatite C Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Transfus Med Assunto da revista: HEMATOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doadores de Sangue / Hepatite C Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Transfus Med Assunto da revista: HEMATOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil