Hippocampal cellular functional organization for fear memory: Effects of sleep.

ABSTRACT

Memory is vital to our daily existence. Although a large number of studies have suggested that the hippocampus is dedicated to long-term memory, understanding how memory is anatomically encoded within the hippocampal neuronal network is still lacking. Previously our laboratory showed that hippocampal pyramidal cells are organized in cell clusters to encode both spatial and episodic memory. Based on these findings, we hypothesized that "cluster-type" is a functional organization principal in the hippocampus to encode all types of memory. Here, we tested whether contextual fear, another hippocampus-dependent memory, is also organized in cell clusters. We further investigated the possibility that post-learning sleep may affect functional organization. Cluster formation was examined by assessing the topographic localization of active cells using immediate early gene (IEG, Zif268) imaging methods. The first experiment provides evidence of a cluster-type organization in the hippocampus for fear memory by showing a spatial distribution of adjacent Zif268 positive cells. Exposure to the context itself, without electric shocks, induced a similar cellular formation; however, the degree of clustering was significantly lower. The second experiment provides evidence that sleep plays a role in the refinement and long-term stability of the clusters. The present results confirm the existence of a cluster-type topographic functional neuronal organization in the hippocampus for memory, and further suggest that post-learning sleep enhances the cluster-type organization.