Isolation and characterization of a full-length rabbit apolipoprotein E cDNA.
Atherosclerosis
; 66(1-2): 125-30, 1987 Jul.
Article
em En
| MEDLINE
| ID: mdl-3632744
ABSTRACT
In order to study the primary structure of rabbit apolipoprotein (apo) E and the regulation of levels of liver apo E mRNA by dietary cholesterol, we have cloned and sequenced a full length rabbit apo E cDNA. DNA sequence analyses suggests that rabbit apo E is synthesized with an additional 18 amino acids as the prepeptide. The mature rabbit apo E contains 293 amino acids with a calculated molecular weight of 33,528. It has a 76% amino acid sequence homology with human apo E. Northern blot analyses showed that rabbit apo E mRNA is about 1200 nucleotides in length. Using mRNA dot blot analyses, we found that dietary cholesterol has no effect on the level of apo E mRNA in rabbit liver. We conclude that the elevated levels of plasma apo E in rabbits fed a cholesterol-rich diet is not a result of an increase of levels of apo E mRNA in the liver.
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Base de dados:
MEDLINE
Assunto principal:
Apolipoproteínas E
/
Coelhos
/
DNA
Limite:
Animals
Idioma:
En
Revista:
Atherosclerosis
Ano de publicação:
1987
Tipo de documento:
Article