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The traditional Chinese medicine Qiangjing tablet prevents blood-testis barrier injury induced by CdCl2 through the PI3K/Akt/Rictor signaling pathway.
Shen, Yifeng; You, Yaodong; Zhu, Kun; Li, Guangsen; Huang, Xiaopeng; Chen, Diang; Yang, Fang; Dong, Liang; Li, Junjun; Yu, Xujun.
Afiliação
  • Shen Y; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • You Y; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Zhu K; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Li G; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Huang X; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Chen D; TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Yang F; Reproductive & Women-Children Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Dong L; Reproductive & Women-Children Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Li J; Chengdu Fifth People's Hospital/The Fifth People's Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
  • Yu X; Chengdu Fifth People's Hospital/The Fifth People's Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Environ Toxicol ; 38(3): 591-603, 2023 Mar.
Article em En | MEDLINE | ID: mdl-36370150
OBJECTIVE: Environmental contaminants such as cadmium (Cd) may have a deleterious impact on sperm and reduce male fertility by compromising the blood-testis barrier (BTB). Hence, the effects of the traditional Chinese medicine Qiangjing tablet (QJP) on sperm quality and BTB alterations induced by Cd in mouse testes were examined. METHODS: Adult KM mice challenged with Cd chloride were examined, QJP was administered to mice as an oral drug by gavage, and the experiments lasted 2 weeks. Testicular and epididymal weights, sperm quality, anti-sperm antibodies (AsAb), hormone levels, and histology were evaluated. Changes in the levels of N-cadherin, occludin, ZO-1, claudin-11, F-actin, and ß-tubulin and their mRNAs were evaluated. The effects of QJP on the PI3K/Akt/Rictor pathway were evaluated. RESULTS: CdCl2 decreased reproductive organ weight, sperm quality, and testosterone (T) levels; increased AsAb, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels; induced structural damage in testicles with BTB disruption; increased BTB permeability; and decreased N-cadherin, occludin, ZO-1, claudin-11, F-actin, and ß-tubulin expression. After treatment, QJP blocked the effects of Cd on reproductive organ weight, sperm quality, and T; mitigated germinal epithelium compartment alterations; decreased AsAb, FSH, and LH levels; and preserved BTB ultrastructure and function. In addition, QJP induced increases in N-cadherin, occludin, ZO-1, claudin-11, F-actin, and ß-tubulin levels and the expression of their mRNAs through the PI3K/Akt/Rictor pathway. After the application of JRAB2011, the levels of a specific mTORC2 suppressor, Rictor, and the BTB-protective effect of QJP were greatly reduced. CONCLUSIONS: We demonstrated the effect of QJP against Cd-induced damage to the BTB, and the results indicate that QJP may play a significant role in opposing the effects of Cd through the PI3K/Akt/Rictor pathway.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Barreira Hematotesticular / Fosfatidilinositol 3-Quinases Limite: Animals Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Barreira Hematotesticular / Fosfatidilinositol 3-Quinases Limite: Animals Idioma: En Revista: Environ Toxicol Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China