Harnessing plant viruses in the metagenomics era: from the development of infectious clones to applications.
Trends Plant Sci
; 28(3): 297-311, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36379846
ABSTRACT
Recent metagenomic studies which focused on virus characterization in the entire plant environment have revealed a remarkable viral diversity in plants. The exponential discovery of viruses also requires the concomitant implementation of high-throughput methods to perform their functional characterization. Despite several limitations, the development of viral infectious clones remains a method of choice to understand virus biology, their role in the phytobiome, and plant resilience. Here, we review the latest approaches for efficient characterization of plant viruses and technical advances built on high-throughput sequencing and synthetic biology to streamline assembly of viral infectious clones. We then discuss the applications of plant viral vectors in fundamental and applied plant research as well as their technical and regulatory limitations, and we propose strategies for their safer field applications.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
/
Metagenômica
Idioma:
En
Revista:
Trends Plant Sci
Assunto da revista:
BOTANICA
Ano de publicação:
2023
Tipo de documento:
Article