Platelet function of whole blood after short-term cold storage: A prospective in vitro observational study.
Transfusion
; 63(2): 384-392, 2023 02.
Article
em En
| MEDLINE
| ID: mdl-36477860
ABSTRACT
BACKGROUND:
There is no standardized storage temperature of whole blood for acute normovolemic hemodilution (ANH). STUDY DESIGN ANDMETHODS:
We conducted a prospective observational study to examine the difference in platelet function between short-term whole blood storage at 4 and 22°C. Venous blood (40 ml) was collected from seven healthy subjects who gave prior written consent. The samples were divided into three groups before storage (group Pre), cold (4°C) storage (group C), and room temperature (22°C) storage (group R). Groups C and R were tested after 6 h of blood storage. Platelet aggregability, platelet factor 4 (PF4), ß-thromboglobulin (ß-TG), P-selectin expression, pH, PO2 , PCO2 , glucose, lactate, blood count, and thromboelastography (TEG) parameters were measured. The percentage change in each parameter in groups C and R was calculated using the value in group Pre as a reference. These data were then compared between groups C and R using a Wilcoxon matched pairs test. p < 0.05 was considered to be statistically significant.RESULTS:
Compared with group R, group C showed significantly higher platelet aggregability with adenosine diphosphate (ADP) 2, 4, and 6 µM (all p = 0.016) and collagen 1 µg/ml (p = 0.047) stimulation, and significantly lower PF4 and ß-TG elevation (both p = 0.031), glucose consumption (p = 0.031), and lactate production (p = 0.016). The ADP channel in TEG showed a significant increase in platelet aggregation rate in group C compared to group R.DISCUSSION:
Cold storage of whole blood in ANH may provide improved storage conditions for platelets and contribute to improved hemostasis compared to room temperature storage.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Hemostasia
Tipo de estudo:
Observational_studies
Limite:
Humans
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Japão