Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae.
Biotechnol Lett
; 45(2): 255-262, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36550338
ABSTRACT
S-Adenosylmethionine (SAM) is a crucial small-molecule metabolite widely used in food and medicine. The development of high-throughput biosensors for SAM biosynthesis can significantly improve the titer of SAM. This paper constructed a synthetic transcription factor (TF)-based biosensor for SAM detecting in Saccharomyces cerevisiae. The synthetic TF, named MetJ-hER-VP16, consists of an Escherichia coli-derived DNA-binding domain MetJ, GS linker, the human estrogen receptor binding domain hER, and the viral activation domain VP16. The synthetic biosensor is capable of sensing SAM in a dose-dependent manner with fluorescence as the output. Additionally, it is tightly regulated by the inducer SAM and ß-estradiol, which means that the fluorescence output is only available when both are present together. The synthetic SAM biosensor could potentially be applied for high-throughput metabolic engineering and is expected to improve SAM production.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
S-Adenosilmetionina
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Saccharomyces cerevisiae
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Fatores de Transcrição
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Técnicas Biossensoriais
Limite:
Humans
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2023
Tipo de documento:
Article