New synthetic silver-doped ZnO nanorods trigger cytotoxicity in MCF-7 through apoptosis and antimicrobial activity.
J Biomol Struct Dyn
; 41(20): 11193-11203, 2023 12.
Article
em En
| MEDLINE
| ID: mdl-36571482
ABSTRACT
The structural, composition, and molecular interaction of silver integrated zinc oxide (20 wt.% Ag/ZnO) were investigated by X-ray diffraction (XRD), energy dispersive X-ray analysis (EDX), and Fourier transforms infrared (FTIR) spectrum. The XRD analysis showed the polycrystalline of small crystallite size, whereas the EDX spectrum confirmed the purity of the nanocomposite. The FTIR spectrum indicated the presence of Ag-Zn-O stretching vibration at 1034 cm-1. SEM and TEM images identified the surface morphology and particle size, indicating that Ag/ZnO of nanorods linked with spherical-like shapes. The nanorods of an average length of â¼110 nm and an average diameter of â¼10 nm. The optical characteristics showed a direct transition of electrons through an energy gap in the 3.30 eV-3.60 eV. The tested nanocomposite exhibited potent cytotoxicity against MCF-7 cells with an IC50 value of 0.26 µg/ml with cell growth inhibition by 97.3% at the highest concentration compared to Doxorubicin (IC50=6.72 µg/ml). It significantly stimulated total apoptotic breast cancer cell death by 51-fold (32.16% compared to 0.63 for the control), arresting the cell progression at the G1 phase. For further validation of apoptotic activity, the tested Ag/ZnO-NP upregulated the proapoptotic genes and down-regulated the anti-apoptotic gene. Moreover, a molecular docking study highlighted the binding disposition of the nanocomposite as Bcl-2 inhibitors. Additionally, Ag/ZnO-NP exhibited potent antimicrobial activity. Hence, the synthesized nanocomposite can serve as an antimicrobial and cytotoxic agent through apoptosis-induction and could be developed as a biologically active nanocomposite.Communicated by Ramaswamy H. Sarma.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Óxido de Zinco
/
Nanotubos
/
Anti-Infecciosos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Biomol Struct Dyn
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Egito