Genetic dissection of intercellular interactions in vivo by membrane-permeable protein.
Proc Natl Acad Sci U S A
; 120(1): e2120582120, 2023 01 03.
Article
em En
| MEDLINE
| ID: mdl-36574652
ABSTRACT
Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically labeled endothelial cells surrounding a special population of hepatocytes in adult mice and revealed their distinct gene signatures. Our results highlight the potential of CILP as a platform to reveal cell-cell interactions and communications in vivo.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células Endoteliais
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China