Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donors.
Cell Rep Methods
; 2(12): 100350, 2022 12 19.
Article
em En
| MEDLINE
| ID: mdl-36590684
ABSTRACT
Gamma-delta (γδ) T cells contribute to the pathology of many immune-related diseases; however, no ex vivo assays to study their activities are currently available. Here, we established a methodology to characterize human allergen-reactive γδ T cells in peripheral blood using an activation-induced marker assay targeting upregulated 4-1BB and CD69 expression. Broad and reproducible ex vivo allergen-reactive γδ T cell responses were detected in donors sensitized to mouse, cockroach, house dust mite, and timothy grass, but the response did not differ from that in non-allergic participants. The reactivity to 4 different allergen extracts was readily detected in 54.2%-100% of allergic subjects in a donor- and allergen-specific pattern and was abrogated by T cell receptor (TCR) blocking. Analysis of CD40L upregulation and intracellular cytokine staining revealed a T helper type 1 (Th1)-polarized response against mouse and cockroach extract stimulation. These results support the existence of allergen-reactive γδ T cells and their potential use in rebalancing dysregulated Th2 responses in allergic diseases.
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1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos Intraepiteliais
/
Hipersensibilidade
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell Rep Methods
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos