Determination of Secondary Structure of Proteins by Nanoinfrared Spectroscopy.
Anal Chem
; 95(2): 621-627, 2023 01 17.
Article
em En
| MEDLINE
| ID: mdl-36598929
ABSTRACT
Nanoscale infrared spectroscopy (AFMIR) is becoming an important tool for the analysis of biological sample, in particular protein assemblies, at the nanoscale level. While the amide I band is usually used to determine the secondary structure of proteins in Fourier transform infrared spectroscopy, no tool has been developed so far for AFMIR. The paper introduces a method for the study of secondary structure of protein based on a protein library of 38 well-characterized proteins. Ascending stepwise linear regression (ASLR) and partial least square (PLS) regression were used to correlate spectrum characteristic bands with the major secondary structures (α-helixes and ß-sheets). ASLR appears to provide better results than PLS. The secondary structure predictions are characterized by a root mean square standard error in a cross validation of 6.39% for α-helixes and 6.23% for ß-sheets.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Amidas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Bélgica