Green synthesis of silica-coated magnetic nanocarriers for simultaneous purification-immobilization of ß-1,3-xylanase.
Int J Biol Macromol
; 233: 123223, 2023 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-36639070
ABSTRACT
Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5-120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM, XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of ß-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability, which would be promising in algae biomass bioconversion as well as other diverse applications.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas
/
Nanopartículas de Magnetita
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Porto Rico