Menin "reads" H3K79me2 mark in a nucleosomal context.
Science
; 379(6633): 717-723, 2023 02 17.
Article
em En
| MEDLINE
| ID: mdl-36795828
ABSTRACT
Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this histone mark is translated into downstream effects remains poorly understood owing to a lack of knowledge about its readers. We developed a nucleosome-based photoaffinity probe to capture proteins that recognize H3K79 dimethylation (H3K79me2) in a nucleosomal context. In combination with a quantitative proteomics approach, this probe identified menin as a H3K79me2 reader. A cryo-electron microscopy structure of menin bound to an H3K79me2 nucleosome revealed that menin engages with the nucleosome using its fingers and palm domains and recognizes the methylation mark through a π-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
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Nucleossomos
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Proteínas Proto-Oncogênicas
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Epigênese Genética
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Lisina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China