Fast and sensitive GCaMP calcium indicators for neuronal imaging.
J Physiol
; 602(8): 1595-1604, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-36811153
ABSTRACT
We review the principles of development and deployment of genetically encoded calcium indicators (GECIs) for the detection of neural activity. Our focus is on the popular GCaMP family of green GECIs, culminating in the recent release of the jGCaMP8 sensors, with dramatically improved kinetics relative to previous generations. We summarize the properties of GECIs in multiple colour channels (blue, cyan, green, yellow, red, far-red) and highlight areas for further improvement. With their low-millisecond rise-times, the jGCaMP8 indicators allow new classes of experiments following neural activity in time frames approaching the underlying computations.
Texto completo:
1
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Physiol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos