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Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved.
Dong, Qixuan; Guo, Yajie; Yuan, Junhua; Zhong, Shuping; Ni, Hao; Liu, Jingyi; Zhang, Mengzhen; Sun, Jiaqi; Yuan, Shuqi; Yu, Huan; Zhong, Yuxu; Jiang, Qixiao.
Afiliação
  • Dong Q; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Guo Y; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Yuan J; Department of Special Medicine, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Zhong S; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Ni H; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Liu J; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Zhang M; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Sun J; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Yuan S; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Yu H; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China.
  • Zhong Y; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing, China. Electronic address: yuxuzhong2008@aliyun.com.
  • Jiang Q; Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China. Electronic address: jiangq@qdu.edu.cn.
Ecotoxicol Environ Saf ; 253: 114671, 2023 Mar 15.
Article em En | MEDLINE | ID: mdl-36822062
ABSTRACT
Hexafluoropropylene oxide tetramer acid (HFPO-TeA) is an emerging environmental contaminant, with environmental presence but limited toxicological information. To investigate its potential developmental toxicities, various doses of HFPO-TeA exposure were achieved in chicken embryos via air cell injection, and the exposed embryos were incubated until hatch. Within 24 h of hatch, the hatchling chickens were assessed with electrocardiography and histopathology for toxicological evaluation. For mechanistic investigation, in ovo silencing of PPARα was achieved via lentivirus microinjection, then the morphological/functional endpoints along with protein expression levels of PPARα-regulated genes were assessed. HFPO-TeA exposure in chicken embryo resulted in developmental cardiotoxicity and hepatotoxicity. Specifically, decreased right ventricular wall thickness, increased heart rate and hepatic steatosis were observed, whereas silencing of PPARα resulted in alleviation of observed toxicities. Western blotting for EHHADH and FABPs suggested that developmental exposure to HFPO-TeA effectively increased the expression levels of both targets in hatchling chicken heart and liver tissue samples, while PPARα silencing prevented such changes, suggesting that PPARα and its downstream genes are playing critical roles in HFPO-TeA induced developmental toxicities.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Galinhas / Fluorocarbonos Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Galinhas / Fluorocarbonos Limite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China