Visualization of α-synuclein trafficking via nanogold labeling and electron microscopy.
STAR Protoc
; 4(1): 102113, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36861831
ABSTRACT
There is conflicting evidence regarding the mechanisms of α-synuclein internalization, and its trafficking itinerary following cellular entry remains largely unknown. To examine these issues, we describe steps for coupling α-synuclein preformed fibrils (PFFs) to nanogold beads and their subsequent characterization by electron microscopy (EM). Then we describe the uptake of conjugated PFFs by U2OS cells plated on Permanox 8-well chamber slides. This process eliminates the reliance on antibody specificity and the need to employ complex immunoEM staining protocols. For complete details on the use and execution of this protocol, please refer to Bayati et al. (2022).1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Alfa-Sinucleína
/
Neurônios
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article