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Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors.
Lane-Reticker, Sarah Kate; Kessler, Emily A; Muscato, Audrey J; Kim, Sarah Y; Doench, John G; Yates, Kathleen B; Manguso, Robert T; Dubrot, Juan.
Afiliação
  • Lane-Reticker SK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Kessler EA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Muscato AJ; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Kim SY; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Doench JG; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Yates KB; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Center for Cancer Research, Massachusetts General Hospital, Boston, MA 02114, USA.
  • Manguso RT; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Center for Cancer Research, Massachusetts General Hospital, Boston, MA 02114, USA. Electronic address: rmanguso@broadinstitute.org.
  • Dubrot J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Solid Tumors Program, Division of Oncology, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain. Electronic address: jdubrot@unav.es.
STAR Protoc ; 4(1): 102082, 2023 03 17.
Article em En | MEDLINE | ID: mdl-36861834
Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in Renca mouse model. This protocol describes how to conduct an in vivo genetic screen with a sgRNA library and SCAR vectors that can be applied to different cell lines and contexts. For complete details on the use and execution of this protocol, please refer to Dubrot et al. (2021).1.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / RNA Guia de Sistemas CRISPR-Cas Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / RNA Guia de Sistemas CRISPR-Cas Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos