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Loss of peroxisomal NAD kinase 3 (NADK3) affects photorespiration metabolism in Arabidopsis.
Suzuki, Shota; Tanaka, Daimu; Miyagi, Atsuko; Takahara, Kentaro; Kono, Masaru; Noguchi, Ko; Ishikawa, Toshiki; Nagano, Minoru; Yamaguchi, Masatoshi; Kawai-Yamada, Maki.
Afiliação
  • Suzuki S; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan.
  • Tanaka D; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan.
  • Miyagi A; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan; Faculty of Agriculture, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan.
  • Takahara K; Institute of Molecular and Cellular Biosciences, the University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan.
  • Kono M; Graduate School of Science, the University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
  • Chaomurilege; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan.
  • Noguchi K; School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
  • Ishikawa T; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan.
  • Nagano M; College of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga, 525-8577, Japan.
  • Yamaguchi M; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan.
  • Kawai-Yamada M; Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama-city, Saitama, 338-8570, Japan. Electronic address: mkawai@mail.saitama-u.ac.jp.
J Plant Physiol ; 283: 153950, 2023 Apr.
Article em En | MEDLINE | ID: mdl-36889102
ABSTRACT
Nicotinamide adenine dinucleotides (NAD+ and NADP+) are electron mediators involved in various metabolic pathways. NADP(H) are produced by NAD kinase (NADK) through the phosphorylation of NAD(H). The Arabidopsis NADK3 (AtNADK3) is reported to preferentially phosphorylate NADH to NADPH and is localized in the peroxisome. To elucidate the biological function of AtNADK3 in Arabidopsis, we compared metabolites of nadk1, nadk2 and nadk3 Arabidopsis T-DNA inserted mutants. Metabolome analysis revealed that glycine and serine, which are intermediate metabolites of photorespiration, both increased in the nadk3 mutants. Plants grown for 6 weeks under short-day conditions showed increased NAD(H), indicating a decrease in the phosphorylation ratio in the NAD(P)(H) equilibrium. Furthermore, high CO2 (0.15%) treatment induced a decrease in glycine and serine in nadk3 mutants. The nadk3 showed a significant decrease in post-illumination CO2 burst, suggesting that the photorespiratory flux was disrupted in the nadk3 mutant. In addition, an increase in CO2 compensation points and a decrease in CO2 assimilation rate were observed in the nadk3 mutants. These results indicate that the lack of AtNADK3 causes a disruption in the intracellular metabolism, such as in amino acid synthesis and photorespiration.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis Idioma: En Revista: J Plant Physiol Assunto da revista: BOTANICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis Idioma: En Revista: J Plant Physiol Assunto da revista: BOTANICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão