Bioassay for total serum bioactivity of Atractylodes lancea.
J Adv Pharm Technol Res
; 14(1): 51-55, 2023.
Article
em En
| MEDLINE
| ID: mdl-36950462
ABSTRACT
The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/µl) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Adv Pharm Technol Res
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Tailândia