Your browser doesn't support javascript.
loading
Beneficial Effects of Hordenine on a Model of Ulcerative Colitis.
Xu, Zhengguang; Zhang, Qilian; Ding, Ce; Wen, Feifei; Sun, Fang; Liu, Yanzhan; Tao, Chunxue; Yao, Jing.
Afiliação
  • Xu Z; School of Basic Medicine, Jining Medical University, Jining 272067, China.
  • Zhang Q; School of Basic Medicine, Jining Medical University, Jining 272067, China.
  • Ding C; School of Basic Medicine, Weifang Medical University, Weifang 261000, China.
  • Wen F; School of Basic Medicine, Jining Medical University, Jining 272067, China.
  • Sun F; School of Basic Medicine, Jining Medical University, Jining 272067, China.
  • Liu Y; School of Basic Medicine, Jining Medical University, Jining 272067, China.
  • Tao C; Jining Key Laboratory of Pharmacology, Jining Medical University, Jining 272067, China.
  • Yao J; School of Basic Medicine, Jining Medical University, Jining 272067, China.
Molecules ; 28(6)2023 Mar 21.
Article em En | MEDLINE | ID: mdl-36985809
Hordenine, a phenethylamine alkaloid, is found in a variety of plants and exhibits a broad array of biological activities and pharmacological properties, including anti-inflammatory and anti-fibrotic effects. However, the efficacy and underlying mechanisms of hordenine in treating ulcerative colitis (UC) remain unclear. To address this, we examined the therapeutic effects of hordenine on dextran sodium sulphate (DSS)-induced UC by comparing disease activity index (DAI), colon length, secretion of inflammatory factors, and degree of colonic histological lesions across diseased mice that were and were not treated with hordenine. We found that hordenine significantly reduced DAI and levels of pro-inflammatory factors, including interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), and also alleviated colon tissue oedema, colonic lesions, inflammatory cells infiltration and decreased the number of goblet cells. Moreover, in vitro experiments showed that hordenine protected intestinal epithelial barrier function by increasing the expression of tight junction proteins including ZO-1 and occludin, while also promoting the healing of intestinal mucosa. Using immunohistochemistry and western blotting, we demonstrated that hordenine reduced the expression of sphingosine kinase 1 (SPHK1), sphingosine-1-phosphate receptor 1 (S1PR1), and ras-related C3 botulinum toxin substrate 1 (Rac1), and it inhibited the expression of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in colon tissues. Thus, hordenine appears to be effective in UC treatment owing to pharmacological mechanisms that favor mucosal healing and the inhibition of SPHK-1/S1PR1/STAT3 signaling.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Colite Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Colite Ulcerativa / Colite Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China