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Effects of n-3 PUFA supplementation on oocyte in vitro maturation in mice with polycystic ovary syndrome.
Ma, Rujun; Wang, Shuxian; Xue, Mengqi; Zhang, Hong; He, Zhaowanyue; Jueraitetibaike, Kadiliya; Ge, Xie; Chen, Li; Yao, Bing.
Afiliação
  • Ma R; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China.
  • Wang S; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
  • Xue M; Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213000, China.
  • Zhang H; State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China.
  • He Z; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
  • Jueraitetibaike K; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
  • Ge X; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
  • Chen L; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
  • Yao B; Center of Reproductive Medicine, Affiliated Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002, China.
J Ovarian Res ; 16(1): 87, 2023 Apr 29.
Article em En | MEDLINE | ID: mdl-37120599
n-3 PUFAs are classic antioxidant that can be used to treat follicular dysplasia and hyperinsulinemia caused by excessive oxidative stress in PCOS women. To investigate the effect of n-3 PUFA supplementation on the oocyte quality of polycystic ovary syndrome (PCOS) mice during in vitro maturation, a PCOS mouse model was established by dehydroepiandrosterone (DHEA). The GV oocytes of the control and PCOS groups were collected and cultured in vitro with or without n-3 PUFAs. After 14 h, the oocytes were collected. Our data demonstrated that the oocyte maturation rate of PCOS mice significantly increased after the addition of 50 µM n-3 PUFAs. The results of immunofluorescence showed that the abnormal rates of spindles and chromosomes in the PCOS + n-3 PUFA group were lower than those in the PCOS group. The mRNA expression of an antioxidant-related gene (Sirt1) and DNA damage repair genes (Brca1/Msh2) was found to be significantly rescued after n-3 treatment. Additionally, the results of living cell staining showed that the addition of n-3 PUFAs could reduce the levels of reactive oxygen species and mitochondrial superoxide in PCOS oocytes. In conclusion, the addition of 50 µM n-3 PUFAs during the in vitro maturation of PCOS mouse oocytes can improve the maturation rate by reducing the level of oxidative stress and the rate of spindle/chromosome abnormalities, providing valuable support during the IVM process.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Síndrome do Ovário Policístico / Ácidos Graxos Ômega-3 Limite: Animals / Female / Humans Idioma: En Revista: J Ovarian Res Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Síndrome do Ovário Policístico / Ácidos Graxos Ômega-3 Limite: Animals / Female / Humans Idioma: En Revista: J Ovarian Res Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China