Perturbations of gut microbiome and metabolome of pigs infected with Mycoplasma hyorhinis.
J Sci Food Agric
; 103(13): 6219-6232, 2023 Oct.
Article
em En
| MEDLINE
| ID: mdl-37145100
ABSTRACT
BACKGROUND:
Mycoplasma hyorhinis is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a large impact on intestinal microecology. To study the effect of M. hyorhinis infection on gut microbial composition and metabolome profile, pigs were infected with M. hyorhinis. Metagenomic sequencing analysis was performed of fecal samples and a liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis of gut digesta was made.RESULTS:
Pigs infected with M. hyorhinis had enriched Sutterella and Mailhella, and depleted Dechloromonas, Succinatimonas, Campylobacter, Blastocystis, Treponema, and Megasphaera. The pigs infected with M. hyorhinis also had greater abundances of bacterium_0_1xD8_71, Ruminococcus_sp__CAG_353, Firmicutes_bacterium_CAG_194, Firmicutes_bacterium_CAG_534, bacterium_1xD42_87, and lower abundances of Chlamydia_suis, Megasphaera_elsdenii, Treponema_porcinum, Bacteroides_sp__CAG_1060, Faecalibacterium_prausnitzii. Metabolomic analysis revealed that some lipids and lipid-like molecules increased in the small intestine, whereas most lipids and lipid-like molecule metabolites decreased in the large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism, and thiamine metabolism.CONCLUSION:
These findings demonstrate that infection with M. hyorhinis can alter the gut microbial composition and metabolite structure in pigs, which may further affect amino acid metabolism and lipid metabolism in the intestine. © 2023 Society of Chemical Industry.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
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Mycoplasma hyorhinis
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Microbioma Gastrointestinal
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Infecções por Mycoplasma
Limite:
Animals
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China