Cryo-EM for Structure Determination of Mitochondrial Ribosome Samples.
Methods Mol Biol
; 2661: 89-100, 2023.
Article
em En
| MEDLINE
| ID: mdl-37166633
ABSTRACT
Single-particle cryoelectron microscopy (cryo-EM) allows structure determination of large macromolecular complexes from conformationally and compositionally heterogeneous mixtures of particles. This technique has been used to reveal the architecture of the mitochondrial ribosome and to visualize transient states that occur during the translation cycle or during mitoribosome biogenesis. Here, we outline an exemplary workflow for the analysis of single-particle cryo-EM data of human mitoribosome samples. In addition, we provide an example dataset which can be used for training purposes alongside the protocol.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos Mitocondriais
/
Imagem Individual de Molécula
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Alemanha