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GIL: a python package for designing custom indexing primers.
Mateyko, Nicholas; Tariq, Omar; Chen, Xinyi E; Cheney, Will; Salaudeen, Asfar Lathif; Luthra, Ishika; Nikpour, Najmeh; Rafi, Abdul Muntakim; Kamali Deghan, Hadis; Jensen, Cassandra; de Boer, Carl.
Afiliação
  • Mateyko N; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Tariq O; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Chen XE; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Cheney W; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Salaudeen AL; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Luthra I; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Nikpour N; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Rafi AM; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Kamali Deghan H; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • Jensen C; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
  • de Boer C; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Bioinformatics ; 39(6)2023 06 01.
Article em En | MEDLINE | ID: mdl-37208164
ABSTRACT

SUMMARY:

Generate Indexes for Libraries (GIL) is a software tool for generating primers to be used in the production of multiplexed sequencing libraries. GIL can be customized in numerous ways to meet user specifications, including length, sequencing modality, color balancing, and compatibility with existing primers, and produces ordering and demultiplexing-ready outputs. AVAILABILITY AND IMPLEMENTATION GIL is written in Python and is freely available on GitHub under the MIT license https//github.com/de-Boer-Lab/GIL and can be accessed as a web-application implemented in Streamlit at https//dbl-gil.streamlitapp.com.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Software / Primers do DNA Idioma: En Revista: Bioinformatics Assunto da revista: INFORMATICA MEDICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Software / Primers do DNA Idioma: En Revista: Bioinformatics Assunto da revista: INFORMATICA MEDICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá