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Development of a chronic focal equine arteritis virus infection of a male reproductive tract cell line.
Martín-Faivre, Lydie; Gaudaire, Delphine; Laugier, Claire; Bouraïma-Lelong, Hélène; Zientara, Stéphan; Hans, Aymeric.
Afiliação
  • Martín-Faivre L; ANSES Laboratory for Animal Health, Normandy site. PhEED Unit, Goustranville, France. Electronic address: lydie.martin@inserm.fr.
  • Gaudaire D; ANSES Laboratory for Animal Health, Normandy site. PhEED Unit, Goustranville, France.
  • Laugier C; ANSES Laboratory for Animal Health, Normandy site. PhEED Unit, Goustranville, France.
  • Bouraïma-Lelong H; Normandie Université, UNICAEN, INRA, OeReCa, 14000 Caen, France.
  • Zientara S; Université Paris-Est, ANSES, Maisons-Alfort Laboratory for Animal Health, UMR Virology ANSES, INRAE, ENVA, Maisons-Alfort, France.
  • Hans A; ANSES Laboratory for Animal Health, Normandy site. PhEED Unit, Goustranville, France.
J Virol Methods ; 319: 114756, 2023 09.
Article em En | MEDLINE | ID: mdl-37268046
Equine arteritis virus (EAV) is an Alphaarterivirus (family Arteriviridae, order Nidovirales) that frequently causes an influenza-like illness in adult horses, but can also cause the abortions in mares and death of newborn foals. Once primary infection has been established, EAV can persist in the reproductive tract of some stallions. However, the mechanisms enabling this persistence, which depends on testosterone, remain largely unknown. We aimed to establish an in vitro model of non-cytopathic EAV infection to study viral persistence. In this work, we infected several cell lines originating from the male reproductive tract of different species. EAV infection was fully cytopathic for 92BR (donkey cells) and DDT1 MF-2 (hamster cells) cells, and less cytopathic for PC-3 cells (human cells); ST cells (porcine cells) seemed to eliminate the virus; LNCaP (human cells) and GC-1 spg (murine cells) cells were not permissive to EAV infection; finally, TM3 cells (murine cells) were permissive to EAV infection without any overt cytopathic effects. Infected TM3 cells can be maintained at least 7 days in culture without any subculture. They can also be subcultured over 39 days (subculturing them at 1:2 the first time at 5 dpi and then every 2-3 days), but in this case, the percentage of infected cells remains low. Infected TM3 cells may therefore provide a new model to study the host-pathogen interactions and to help determine the mechanisms involved in EAV persistence in stallion reproductive tract.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Arterivirus / Equartevirus / Doenças dos Cavalos Limite: Animals / Female / Humans / Male / Pregnancy Idioma: En Revista: J Virol Methods Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Arterivirus / Equartevirus / Doenças dos Cavalos Limite: Animals / Female / Humans / Male / Pregnancy Idioma: En Revista: J Virol Methods Ano de publicação: 2023 Tipo de documento: Article