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Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C.
Loy, Conor J; Sotomayor-Gonzalez, Alicia; Servellita, Venice; Nguyen, Jenny; Lenz, Joan; Bhattacharya, Sanchita; Williams, Meagan E; Cheng, Alexandre P; Bliss, Andrew; Saldhi, Prachi; Brazer, Noah; Streithorst, Jessica; Suslovic, William; Hsieh, Charlotte J; Bahar, Burak; Wood, Nathan; Foresythe, Abiodun; Gliwa, Amelia; Bhakta, Kushmita; Perez, Maria A; Hussaini, Laila; Anderson, Evan J; Chahroudi, Ann; Delaney, Meghan; Butte, Atul J; DeBiasi, Roberta L; Rostad, Christina A; De Vlaminck, Iwijn; Chiu, Charles Y.
Afiliação
  • Loy CJ; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Sotomayor-Gonzalez A; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Servellita V; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Nguyen J; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Lenz J; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Bhattacharya S; Bakar Computational Health Sciences Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Williams ME; Children's National Hospital, Washington, DC 20010, USA.
  • Cheng AP; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Bliss A; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA.
  • Saldhi P; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Brazer N; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Streithorst J; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Suslovic W; Children's National Hospital, Washington, DC 20010, USA.
  • Hsieh CJ; Division of Pediatric Infectious Diseases and Global Health, Department of Pediatrics, University of California San Francisco, Oakland, CA 94609.
  • Bahar B; Children's National Hospital, Washington, DC 20010, USA.
  • Wood N; UCSF Benioff Children's Hospital, Oakland, CA 94609, USA.
  • Foresythe A; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Gliwa A; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Bhakta K; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
  • Perez MA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
  • Hussaini L; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
  • Anderson EJ; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA; Department of Medicine, Emory University School of Medicine, Atlanta, GA 30307, USA.
  • Chahroudi A; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
  • Delaney M; Children's National Hospital, Washington, DC 20010, USA; The George Washington University School of Medicine, Washington, DC 20052, USA.
  • Butte AJ; Bakar Computational Health Sciences Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
  • DeBiasi RL; Children's National Hospital, Washington, DC 20010, USA; The George Washington University School of Medicine, Washington, DC 20052, USA.
  • Rostad CA; Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA; Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
  • De Vlaminck I; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA. Electronic address: id93@cornell.edu.
  • Chiu CY; Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: charles.chiu@ucsf.edu.
Cell Rep Med ; 4(6): 101034, 2023 06 20.
Article em En | MEDLINE | ID: mdl-37279751
ABSTRACT
Differential host responses in coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome in children (MIS-C) remain poorly characterized. Here, we use next-generation sequencing to longitudinally analyze blood samples from pediatric patients with COVID-19 or MIS-C across three hospitals. Profiling of plasma cell-free nucleic acids uncovers distinct signatures of cell injury and death between COVID-19 and MIS-C, with increased multiorgan involvement in MIS-C encompassing diverse cell types, including endothelial and neuronal cells, and an enrichment of pyroptosis-related genes. Whole-blood RNA profiling reveals upregulation of similar pro-inflammatory pathways in COVID-19 and MIS-C but also MIS-C-specific downregulation of T cell-associated pathways. Profiling of plasma cell-free RNA and whole-blood RNA in paired samples yields different but complementary signatures for each disease state. Our work provides a systems-level view of immune responses and tissue damage in COVID-19 and MIS-C and informs future development of new disease biomarkers.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Ácidos Nucleicos Livres / COVID-19 Limite: Child / Humans Idioma: En Revista: Cell Rep Med Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ácidos Nucleicos / Ácidos Nucleicos Livres / COVID-19 Limite: Child / Humans Idioma: En Revista: Cell Rep Med Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos