DeepCLEM: automated registration for correlative light and electron microscopy using deep learning.
F1000Res
; 9: 1275, 2020.
Article
em En
| MEDLINE
| ID: mdl-37397873
ABSTRACT
In correlative light and electron microscopy (CLEM), the fluorescent images must be registered to the EM images with high precision. Due to the different contrast of EM and fluorescence images, automated correlation-based alignment is not directly possible, and registration is often done by hand using a fluorescent stain, or semi-automatically with fiducial markers. We introduce "DeepCLEM", a fully automated CLEM registration workflow. A convolutional neural network predicts the fluorescent signal from the EM images, which is then automatically registered to the experimentally measured chromatin signal from the sample using correlation-based alignment. The complete workflow is available as a Fiji plugin and could in principle be adapted for other imaging modalities as well as for 3D stacks.
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Base de dados:
MEDLINE
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Aprendizado Profundo
Idioma:
En
Revista:
F1000Res
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Alemanha